New tool in CRISPR genome editing, Cpf1, proved its marked specificity, produced a targeted mutant mouse

Two studies outline Cpf1’s superiority as a precise genome editing tool with no unintended mutations. It requires only a single RNA that CRISPR RNA assembly is simpler; its staggered cleavage patterns may facilitate substituting existing DNA with desired sequences; and it recognizes thymidine-rich DNA sequences, which has been less explored than the guanosine-rich sequences recognized by Cas9.